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Parameter

Absorbance and Concentration Measurement

Absorbance links the light a solution takes up to the concentration of what is dissolved in it. Colorimeters work with fixed filters; spectrophotometers select any wavelength across the visible range.

Spectrophotometers
340 – 1020 nm
Colorimeters
400 – 700 nm
Modes
%T · Abs · Conc
Bandwidth
2 nm
Instruments

Recommended instruments

All spectrophotometers →
Method

How it is measured

01

Select

Choose a filter or set the wavelength where the analyte absorbs most.

02

Zero

Set 100 %T with a blank in the cuvette.

03

Read

Absorbance of the sample gives concentration through a standard or K factor.

Applications

Where it is measured

Related parameters
Questions

Frequently asked

Colorimeter or spectrophotometer?

A colorimeter is simple and economical for methods with a fixed filter. A spectrophotometer sets any wavelength, which suits method development and a wider range of tests.

Are cuvettes supplied?

Spectrophotometers ship with two 10 mm glass cuvettes; the K-factor colorimeter ships with five glass sample tubes.

Application support

Comparing colorimeters and spectrophotometers?

Share the tests you run. A specialist will recommend the right instrument.

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