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Fluorescence Measurement
Some compounds re-emit light when excited at a shorter wavelength. Measuring that emission detects them at concentrations too low for absorbance, with quinine as the classic reference.
- Ranges
- 4
- Filters
- 5
- Source
- 12 V · 50 W halogen
- Display
- 3-digit LED
Instruments
Photo fluorometers →Recommended instruments
Method
How it is measured
01
Excite
A primary filter passes the excitation wavelength to the sample.
02
Isolate
A secondary filter passes only the emitted light to the detector.
03
Quantify
Emission is read against a standard such as quinine sulphate.
Applications
Where it is measured
Questions
Frequently asked
Why use fluorescence instead of absorbance?
Fluorescence is measured against a dark background, so it detects much lower concentrations of compounds that fluoresce.
Which filters are supplied?
A set of five primary and secondary filters ships with the instrument.
Application support
Working with fluorescent analytes?
Describe the sample and the method. A specialist will confirm the right set-up.
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